{"added":null,"apiVersion":1,"behindSince":null,"buildDepends":[],"categories":["biology"],"comment":"Primer3 helps to choose primers for PCR reactions","compare":"/compare/primer3/index.json","dependentCount":null,"description":"\"Primer3 is a complete rewrite of the original PRIMER program\n(Primer 0.5), written by Steve Lincoln, Mark Daly, and Eric\nLander.  See DIFFERENCES FROM EARLIER VERSIONS for a discussion\nof how Primer3 differs from its predecessors, Primer 0.5 and\nPrimer v2.\n\nPrimer3 picks primers for PCR reactions, considering as criteria:\n\no oligonucleotide melting temperature, size, GC content,\n  and primer-dimer possibilities,\no PCR product size,\no positional constraints within the source sequence, and\no miscellaneous other constraints.\n\nAll of these criteria are user-specifiable as constraints, and\nsome are specifiable as terms in an objective function that\ncharacterizes an optimal primer pair.\"\n- from the README file","flavors":[],"html":"https://daemonports.org/dragonfly/biology/primer3/","kind":"port","lastUpdate":null,"license":["GPLv2"],"maintainer":"ports@FreeBSD.org","name":"primer3","newest":true,"newestVersion":"2.3.6","noVulnData":false,"origin":"biology/primer3","otherSystems":[{"maintainer":"ports@FreeBSD.org","newest":true,"origin":"biology/primer3","path":"/biology/primer3/","system":"FreeBSD","version":"2.3.6"},{"maintainer":"pkgsrc-users@NetBSD.org","newest":true,"origin":"biology/primer3","path":"/netbsd/biology/primer3/","system":"NetBSD","updated":"2026-08-04","version":"2.3.6"},{"maintainer":"ports@FreeBSD.org","newest":true,"noVulnData":true,"origin":"biology/primer3","path":"","system":"HardenedBSD","version":"2.3.6"},{"maintainer":"ports@FreeBSD.org","newest":true,"noVulnData":true,"origin":"biology/primer3","path":"","system":"GhostBSD","version":"2.3.6"}],"packages":null,"pkgName":"primer3-2.3.6","related":null,"repository":"DPorts","requiredBy":[],"requiredByCount":0,"runDepends":[],"status":null,"system":"dragonfly","systemLabel":"DragonFly","version":"2.3.6","versions":null,"vulnerabilities":null,"www":"http://www-genome.wi.mit.edu/genome_software/other/primer3.html"}
