{"added":null,"apiVersion":1,"behindSince":null,"buildDepends":[],"categories":["biology"],"comment":"Design PCR primers","compare":"/compare/primer3/index.json","dependentCount":null,"description":"Primer3 is a widely used program for designing PCR primers (PCR =\n\"Polymerase Chain Reaction\").  PCR is an essential and ubiquitous tool\nin genetics and molecular biology.  Primer3 can also design\nhybridization probes and sequencing primers.\n\nPrimer3 picks primers for PCR reactions, considering as criteria: o\n\n- oligonucleotide melting temperature, size, GC content, and\n  primer-dimer possibilities,\n- PCR product size,\n- positional constraints within the source (template) sequence,\n- possibilities for ectopic priming (amplifying the wrong sequence), and\n- many other constraints.\n\nAll of these criteria are user-specifiable as constraints, and some\nare specifiable as terms in an objective function that characterizes\nan optimal primer pair.\n\nWhitehead Institute for Biomedical Research provides a basic web-based\nfront end to primer3 named primer3web at http://fokker.wi.mit.edu/\n\nWageningen University Laboratory of Bioinformatics provides a enhanced\nweb-based front end to primer3 named primer3plus at\nhttp://www.bioinformatics.nl/cgi-bin/primer3plus/primer3plus.cgi","flavors":[],"html":"https://daemonports.org/netbsd/biology/primer3/","kind":"port","lastUpdate":"2026-08-04T20:28:42Z","license":["gnu-gpl-v2"],"maintainer":"pkgsrc-users@NetBSD.org","name":"primer3","newest":true,"newestVersion":"2.3.6","noVulnData":false,"origin":"biology/primer3","otherSystems":[{"maintainer":"ports@FreeBSD.org","newest":true,"origin":"biology/primer3","path":"/biology/primer3/","system":"FreeBSD","version":"2.3.6"},{"maintainer":"ports@FreeBSD.org","newest":true,"origin":"biology/primer3","path":"/dragonfly/biology/primer3/","system":"DragonFly","version":"2.3.6"},{"maintainer":"ports@FreeBSD.org","newest":true,"noVulnData":true,"origin":"biology/primer3","path":"","system":"HardenedBSD","version":"2.3.6"},{"maintainer":"ports@FreeBSD.org","newest":true,"noVulnData":true,"origin":"biology/primer3","path":"","system":"GhostBSD","version":"2.3.6"}],"packages":[{"repo":"NetBSD 10.1 x86_64","versions":["2.3.6"]},{"repo":"NetBSD 10.1 aarch64","versions":["2.3.6"]},{"repo":"SmartOS / illumos x86_64","versions":["2.3.6"]},{"repo":"macOS 15 arm64","versions":["-"]}],"pkgName":"primer3-2.3.6","related":null,"repository":"pkgsrc","requiredBy":[],"requiredByCount":0,"runDepends":[],"status":null,"system":"netbsd","systemLabel":"NetBSD","version":"2.3.6","versions":null,"vulnerabilities":null,"www":"https://primer3.sourceforge.net/"}