biology/pooler 1.89 DragonFly

Optimise combinations of primers and minimise the formation of dimers in multiplexed PCR.

Primer Pooler can:

* Check through each proposed pool for combinations that are likely to form dimers

* Automatically move prospective amplicons between proposed pools to reduce dimer formation

* Automatically search the genome sequence to find which amplicons overlap, and place their corresponding primers in separate pools

* Optionally keep pool sizes within a specified range

* Handle thousands of primers without being slow (useful for high-throughput sequencing applications)

* Do all of the above with degenerate primers too.