wip/nanoget 1.16.1.20210320 NetBSD
Extract information from Oxford Nanopore sequencing data
- System
- NetBSD
- Repository
- pkgsrc-wip
- Maintainer
- [email protected]
- License
- mit
- Categories
- wip, biology, python
Description
Nanoget provides functions to extract useful metrics from Oxford Nanopore sequencing reads and alignments.
## Functions
Data can be presented in the following formats, using the following functions:
- A sorted bam file `process_bam(bamfile, threads)`
- A standard fastq file `process_fastq_plain(fastqfile, 'threads')`
- A fastq file with metadata from MinKNOW or Albacore `process_fastq_rich(fastqfile)`
- A sequencing_summary file generated by Albacore `process_summary(sequencing_summary.txt, 'readtype')`
Fastq files can be compressed using gzip, bzip2 or bgzip. The data is returned as a pandas DataFrame with standardized headernames for convenient extraction. The functions perform logging while being called and extracting data.
Install
From pkgsrc-wip (no binary package):
cd /usr/pkgsrc/wip/nanoget && make install clean